General Cell Based Assays
Meso Scale Discovery U-PLEX Mouse IL-22 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-22 Assay Kit is a multiplex immunoassay for measuring mouse interleukin 22 (IL-22) in various samples It uses electrochemiluminescence detection for high sensitivity and precision The kit includes a plate with capture antibodies ECL-tagged detection antibodies calibrators controls and necessary buffers It requires 50 L of sample per well and quantifies IL-22 from 1 5 to 10 000 pg/mL in about 3 5 hours aiding in the study of mucosal immunity tissue regeneration and inflammation
Eurofins Discoverx PathHunter eXpress IL7R/IL2RG Dimerization Assay, 10pl Kit. (93-0997E13CP0L)
The PathHunter IL7R/IL2RG Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-7) induced dimerization of IL7R/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside IL2RG target protein fused to EA. Upon ligand-induced dimerization of IL7R/IL2RG, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCX3CR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0702E2MCP2M)
The PathHunter mCX3CR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CX3CL1, fractalkine) based activation of mCX3CR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCX3CR1 and EA-tagged β-Arrestin-2. Activation of mCX3CR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BDKRB2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0189E2CP0M)
The PathHunter BDKRB2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EpoR-JAK2 Functional Assay, 10-Plate Kit. (93-0900E3CP16L)
The PathHunter EpoR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses EpoR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EpoR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Biolegend Alexa FluorR 647 anti-AMPKalph
Alexa Fluor 647 anti-AMPKα Phospho (Thr172) [A20017A]; Isotype: Mouse IgG1, κ; Reactivity: Human, Mouse; Apps: IHC-P, ICFC; Size: 100 μg
Meso Scale Discovery U-PLEX Human A2M ASY 25 PL
The Meso Scale Discovery U-PLEX Human A2M Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of Alpha-2-Macroglobulin (A2M) in human serum plasma and cell culture supernatants This electrochemiluminescent assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and accurate detection of A2M levels down to sub-pg/mL concentrations The assay requires 50 L sample volume and can be read on MSD instrument platforms This product provides a simple rapid way to quantify A2M levels which can aid research into inflammatory conditions immune function and proteolytic regulation
Meso Scale Discovery R-PLEX Human IL-36 ASY
The Meso Scale Discovery R-PLEX Human IL-36gamma Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of IL-36gamma in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements This assay kit includes a plate coated with capture antibodies detection antibodies labeled with an ECL compound and calibrator and control solutions for generating standard curves and evaluating run validity It allows sensitive reproducible quantification of IL-36gamma levels from human samples
Eurofins Discoverx PathHunter eXpress GPR146 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0365E2ACP1M)
The PathHunter GPR146 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR146 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR146 and EA-tagged β-Arrestin-2. Activation of GPR146 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDG1 Total Internalization Assay, 10pl Kit. (93-0784E1CP28L)
The PathHunter EDG1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of EDG1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged EDG1 and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDG1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress PROKR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0090E2CP2M)
The cAMP Hunter PROKR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PROKR1, a naturally Gs coupled GPCR. Upon activation of PROKR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human DRD2L β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human DRD2L β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure DRD2L (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx InCELL Hunter eXpress MEK1 Kinase Binding Assay, 10pl Kit. (96-0040E15CP0L)
The InCELL Hunter eXpress MEK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of MEK1 via detection of MEK1 levels. This product uses EFC technology. The cell line included overexpresses MEK1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 26 Reagent, 500. (93-0563R26B)
AssayComplete Cell Plating 26 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Meso Scale Discovery SPLEX HUM CTLA4 Kit 25 PL
The Meso Scale Discovery CTLA-4 Discovery Kit is a highly sensitive immunoassay that quantitatively measures soluble CTLA-4 in serum plasma and cell culture supernatants The assay uses MSD s electrochemiluminescent detection technology and provides a lower limit of detection of 0 05 ng/mL The kit includes a 96-well plate coated with capture antibody a detection antibody conjugated to an electrochemiluminescent label and calibrators and controls to generate a standard curve The assay takes approximately 3 5 hours to complete and requires a MSD instrument such as SECTOR Imager QuickPlex or Discovery Workbench to read the plate The kit can test up to 80 samples in duplicate